diff --git a/src/js/_enqueues/wp/customize/base.js b/src/js/_enqueues/wp/customize/base.js
index 4afaa945571f0..0486047b42617 100644
--- a/src/js/_enqueues/wp/customize/base.js
+++ b/src/js/_enqueues/wp/customize/base.js
@@ -985,6 +985,33 @@ window.wp = window.wp || {};
 		return queryParams;
 	};
 
+	/**
+	 * Whether a URL pathname begins with one of the given excluded path prefixes.
+	 *
+	 * @since 7.2.0
+	 * @access public
+	 *
+	 * @alias wp.customize.utils.isExcludedPath
+	 *
+	 * @param {string} pathname        URL pathname to test.
+	 * @param {Array}  [excludedPaths] URL path prefixes, each with a trailing slash.
+	 * @return {boolean} Whether the pathname begins with an excluded path.
+	 */
+	api.utils.isExcludedPath = function isExcludedPath( pathname, excludedPaths ) {
+		var normalizedPathname;
+
+		if ( ! excludedPaths || ! excludedPaths.length ) {
+			return /\/wp-(admin|includes|content)(\/|$)/.test( pathname );
+		}
+
+		// Normalize so '/wp-admin' and '//wp-admin/' both match the '/wp-admin/' prefix.
+		normalizedPathname = ( pathname + '/' ).replace( /\/+/g, '/' );
+
+		return _.some( excludedPaths, function( excludedPath ) {
+			return 0 === normalizedPathname.indexOf( excludedPath );
+		} );
+	};
+
 	/**
 	 * Expose the API publicly on window.wp.customize
 	 *
diff --git a/src/js/_enqueues/wp/customize/controls.js b/src/js/_enqueues/wp/customize/controls.js
index 00123f9141c30..0ca7ceff6d1da 100644
--- a/src/js/_enqueues/wp/customize/controls.js
+++ b/src/js/_enqueues/wp/customize/controls.js
@@ -6534,6 +6534,9 @@
 		 * @param {Array}  params.allowedUrls
 		 * @param {string} params.container   A selector or jQuery element for the preview
 		 *                                    frame to be placed.
+		 * @param {Array}  [params.excludedPaths] URL path prefixes which are not previewable,
+		 *                                        such as the paths for the admin and content
+		 *                                        directories. Added in 7.2.0.
 		 * @param {string} params.form
 		 * @param {string} params.previewUrl  The URL to preview.
 		 * @param {Object} options
@@ -6571,8 +6574,9 @@
 				previewer.refreshBuffer
 			);
 
-			previewer.container   = api.ensure( params.container );
-			previewer.allowedUrls = params.allowedUrls;
+			previewer.container     = api.ensure( params.container );
+			previewer.allowedUrls   = params.allowedUrls;
+			previewer.excludedPaths = params.excludedPaths;
 
 			params.url = window.location.href;
 
@@ -6596,8 +6600,8 @@
 				urlParser = document.createElement( 'a' );
 				urlParser.href = to;
 
-				// Abort if URL is for admin or (static) files in wp-includes or wp-content.
-				if ( /\/wp-(admin|includes|content)(\/|$)/.test( urlParser.pathname ) ) {
+				// Abort if URL is for the admin or (static) files in wp-includes or wp-content.
+				if ( api.utils.isExcludedPath( urlParser.pathname, previewer.excludedPaths ) ) {
 					return null;
 				}
 
@@ -7461,10 +7465,11 @@
 		 * @alias wp.customize.previewer
 		 */
 		api.previewer = new api.Previewer({
-			container:   '#customize-preview',
-			form:        '#customize-controls',
-			previewUrl:  api.settings.url.preview,
-			allowedUrls: api.settings.url.allowed
+			container:     '#customize-preview',
+			form:          '#customize-controls',
+			previewUrl:    api.settings.url.preview,
+			allowedUrls:   api.settings.url.allowed,
+			excludedPaths: api.settings.url.excludedPaths
 		},/** @lends wp.customize.previewer */{
 
 			nonce: api.settings.nonce,
diff --git a/src/js/_enqueues/wp/customize/preview.js b/src/js/_enqueues/wp/customize/preview.js
index 375cd2104ba0f..9ee016329621b 100644
--- a/src/js/_enqueues/wp/customize/preview.js
+++ b/src/js/_enqueues/wp/customize/preview.js
@@ -270,6 +270,7 @@
 	 * Should the supplied link is previewable.
 	 *
 	 * @since 4.7.0
+	 * @since 7.2.0 Non-previewable paths are read from settings instead of being hardcoded.
 	 * @access public
 	 *
 	 * @param {HTMLAnchorElement|HTMLAreaElement} element Link element.
@@ -314,8 +315,8 @@
 			return args.allowAdminAjax;
 		}
 
-		// Disallow links to admin, includes, and content.
-		if ( /\/wp-(admin|includes|content)(\/|$)/.test( element.pathname ) ) {
+		// Disallow links to admin, includes, content, plugins, and uploads paths, which are not previewable.
+		if ( api.utils.isExcludedPath( element.pathname, api.settings.url.excludedPaths ) ) {
 			return false;
 		}
 
diff --git a/src/wp-includes/class-wp-customize-manager.php b/src/wp-includes/class-wp-customize-manager.php
index e298b04efcf90..8dba6e7e098bb 100644
--- a/src/wp-includes/class-wp-customize-manager.php
+++ b/src/wp-includes/class-wp-customize-manager.php
@@ -2169,6 +2169,7 @@ public function customize_preview_settings() {
 				'self'          => $self_url,
 				'allowed'       => array_map( 'sanitize_url', $this->get_allowed_urls() ),
 				'allowedHosts'  => array_unique( $allowed_hosts ),
+				'excludedPaths' => $this->get_excluded_url_paths(),
 				'isCrossDomain' => $this->is_cross_domain(),
 			),
 			'channel'           => $this->messenger_channel,
@@ -4679,6 +4680,52 @@ public function get_allowed_urls() {
 		return $allowed_urls;
 	}
 
+	/**
+	 * Gets URL path prefixes which are excluded from being previewed.
+	 *
+	 * @since 7.2.0
+	 *
+	 * @return string[] URL path prefixes, each with a trailing slash.
+	 */
+	public function get_excluded_url_paths() {
+		$excluded_urls = array(
+			admin_url( '/' ),
+			includes_url( '/' ),
+			content_url( '/' ),
+			plugins_url( '/' ),
+			wp_get_upload_dir()['baseurl'],
+		);
+
+		$parsed_home = wp_parse_url( home_url( '/' ) );
+		$home_host   = strtolower( $parsed_home['host'] ?? '' ) . ':' . ( $parsed_home['port'] ?? '' );
+		$home_path   = trailingslashit( $parsed_home['path'] ?? '/' );
+
+		$excluded_paths = array();
+		foreach ( $excluded_urls as $excluded_url ) {
+			$parsed = wp_parse_url( $excluded_url );
+			if ( ! is_array( $parsed ) ) {
+				continue;
+			}
+
+			// Skip URLs served from another host, such as an uploads URL pointing to a CDN.
+			$host = strtolower( $parsed['host'] ?? '' ) . ':' . ( $parsed['port'] ?? '' );
+			if ( $host !== $home_host ) {
+				continue;
+			}
+
+			$path = trailingslashit( $parsed['path'] ?? '' );
+
+			// A path at or above the home path would exclude the whole site from being previewed.
+			if ( str_starts_with( $home_path, $path ) ) {
+				continue;
+			}
+
+			$excluded_paths[] = $path;
+		}
+
+		return array_values( array_unique( $excluded_paths ) );
+	}
+
 	/**
 	 * Gets messenger channel.
 	 *
@@ -4938,6 +4985,7 @@ public function customize_pane_settings() {
 				'activated'     => sanitize_url( home_url( '/' ) ),
 				'ajax'          => sanitize_url( admin_url( 'admin-ajax.php', 'relative' ) ),
 				'allowed'       => array_map( 'sanitize_url', $this->get_allowed_urls() ),
+				'excludedPaths' => $this->get_excluded_url_paths(),
 				'isCrossDomain' => $this->is_cross_domain(),
 				'home'          => sanitize_url( home_url( '/' ) ),
 				'login'         => sanitize_url( $login_url ),
diff --git a/tests/phpunit/tests/customize/manager.php b/tests/phpunit/tests/customize/manager.php
index bedf0b39b3e95..d25ef59366ec9 100644
--- a/tests/phpunit/tests/customize/manager.php
+++ b/tests/phpunit/tests/customize/manager.php
@@ -2289,6 +2289,134 @@ public function filter_customize_allowed_urls( $urls ) {
 		return $urls;
 	}
 
+	/**
+	 * Test WP_Customize_Manager::get_excluded_url_paths().
+	 *
+	 * @ticket 65030
+	 * @covers WP_Customize_Manager::get_excluded_url_paths
+	 */
+	public function test_get_excluded_url_paths() {
+		$wp_customize = new WP_Customize_Manager();
+
+		$this->assertSame(
+			array(
+				'/wp-admin/',
+				'/wp-includes/',
+				'/wp-content/',
+				'/wp-content/plugins/',
+				'/wp-content/uploads/',
+			),
+			$wp_customize->get_excluded_url_paths()
+		);
+	}
+
+	/**
+	 * Test WP_Customize_Manager::get_excluded_url_paths() for a subdirectory installation.
+	 *
+	 * @ticket 65030
+	 * @covers WP_Customize_Manager::get_excluded_url_paths
+	 */
+	public function test_get_excluded_url_paths_with_subdirectory_install() {
+		$home_url        = trailingslashit( home_url() );
+		$to_subdirectory = static function ( $url ) use ( $home_url ) {
+			return str_replace( $home_url, $home_url . 'wp-content/subsite/', $url );
+		};
+		add_filter( 'admin_url', $to_subdirectory );
+		add_filter( 'includes_url', $to_subdirectory );
+		add_filter( 'content_url', $to_subdirectory );
+		add_filter( 'plugins_url', $to_subdirectory );
+		add_filter(
+			'upload_dir',
+			static function ( $uploads ) use ( $to_subdirectory ) {
+				$uploads['baseurl'] = $to_subdirectory( $uploads['baseurl'] );
+				return $uploads;
+			}
+		);
+
+		$wp_customize = new WP_Customize_Manager();
+
+		// The site's own base path must not be excluded, only the WP directories under it.
+		$this->assertSame(
+			array(
+				'/wp-content/subsite/wp-admin/',
+				'/wp-content/subsite/wp-includes/',
+				'/wp-content/subsite/wp-content/',
+				'/wp-content/subsite/wp-content/plugins/',
+				'/wp-content/subsite/wp-content/uploads/',
+			),
+			$wp_customize->get_excluded_url_paths()
+		);
+	}
+
+	/**
+	 * Test that WP_Customize_Manager::get_excluded_url_paths() never excludes the site root.
+	 *
+	 * @ticket 65030
+	 * @covers WP_Customize_Manager::get_excluded_url_paths
+	 */
+	public function test_get_excluded_url_paths_skips_site_root() {
+		add_filter(
+			'content_url',
+			static function () {
+				return home_url( '/' );
+			}
+		);
+
+		$wp_customize = new WP_Customize_Manager();
+		$excluded     = $wp_customize->get_excluded_url_paths();
+
+		$this->assertNotContains( '/', $excluded );
+		$this->assertNotContains( '', $excluded );
+	}
+
+	/**
+	 * Test that WP_Customize_Manager::get_excluded_url_paths() never excludes an ancestor of the home path.
+	 *
+	 * @ticket 65030
+	 * @covers WP_Customize_Manager::get_excluded_url_paths
+	 */
+	public function test_get_excluded_url_paths_skips_ancestors_of_home_path() {
+		// Simulate a site installed under wp-content whose content directory is the shared parent.
+		$home_url = trailingslashit( home_url() );
+		add_filter(
+			'home_url',
+			static function ( $url ) use ( $home_url ) {
+				return str_replace( $home_url, $home_url . 'wp-content/subsite/', $url );
+			}
+		);
+
+		$wp_customize = new WP_Customize_Manager();
+		$excluded     = $wp_customize->get_excluded_url_paths();
+
+		// Excluding '/wp-content/' would exclude the whole site at '/wp-content/subsite/'.
+		$this->assertNotContains( '/wp-content/', $excluded );
+		$this->assertContains( '/wp-admin/', $excluded );
+	}
+
+	/**
+	 * Test that WP_Customize_Manager::get_excluded_url_paths() skips URLs served from another host.
+	 *
+	 * @ticket 65030
+	 * @covers WP_Customize_Manager::get_excluded_url_paths
+	 */
+	public function test_get_excluded_url_paths_skips_other_hosts() {
+		add_filter(
+			'upload_dir',
+			static function ( $uploads ) {
+				$uploads['baseurl'] = 'https://cdn.example.net/media';
+				return $uploads;
+			}
+		);
+
+		$wp_customize = new WP_Customize_Manager();
+		$excluded     = $wp_customize->get_excluded_url_paths();
+
+		// The CDN path must not block the site's own '/media/' pages, and the CDN itself is not previewable.
+		$this->assertNotContains( '/media/', $excluded );
+		$this->assertNotContains( '/wp-content/uploads/', $excluded );
+		$this->assertContains( '/wp-content/', $excluded );
+	}
+
 	/**
 	 * Test WP_Customize_Manager::doing_ajax().
 	 *
@@ -3147,6 +3275,7 @@ public function test_customize_pane_settings() {
 		$this->assertSame( $autofocus, $data['autofocus'] );
 		$this->assertArrayHasKey( 'save', $data['nonce'] );
 		$this->assertArrayHasKey( 'preview', $data['nonce'] );
+		$this->assertSame( $this->manager->get_excluded_url_paths(), $data['url']['excludedPaths'] );
 
 		$this->assertSameSets(
 			array(
@@ -3218,6 +3347,7 @@ public function test_customize_preview_settings() {
 		$this->assertArrayHasKey( 'changeset', $settings );
 
 		$this->assertArrayHasKey( 'preview', $settings['nonce'] );
+		$this->assertSame( $this->manager->get_excluded_url_paths(), $settings['url']['excludedPaths'] );
 	}
 
 	/**
diff --git a/tests/qunit/wp-admin/js/customize-base.js b/tests/qunit/wp-admin/js/customize-base.js
index bd3535b3bc3b0..16dd6294c72a0 100644
--- a/tests/qunit/wp-admin/js/customize-base.js
+++ b/tests/qunit/wp-admin/js/customize-base.js
@@ -249,4 +249,33 @@ jQuery( function( $ ) {
 		queryParams = wp.customize.utils.parseQueryString( 'a=1&b=' );
 		assert.ok( _.isEqual( queryParams, { 'a': '1', b: '' } ) );
 	} );
+
+	QUnit.module( 'Customize Base: utils.isExcludedPath' );
+	QUnit.test( 'wp.customize.utils.isExcludedPath works', function( assert ) {
+		var excludedPaths = [ '/wp-admin/', '/wp-includes/', '/wp-content/' ];
+
+		// Excluded paths and everything beneath them match.
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-admin/', excludedPaths ) );
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-admin', excludedPaths ), 'bare directory without trailing slash matches' );
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-content/uploads/file.pdf', excludedPaths ) );
+		assert.ok( wp.customize.utils.isExcludedPath( '//wp-admin/', excludedPaths ), 'repeated slashes are collapsed' );
+
+		// Front-end paths do not match.
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/', excludedPaths ) );
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/sample-page/', excludedPaths ) );
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/wp-admin2/', excludedPaths ), 'similarly named directory does not match' );
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/blog/wp-content/', excludedPaths ), 'prefixes are anchored to the start of the path' );
+
+		// Subdirectory install: the site's own base path is not excluded (see #65030).
+		excludedPaths = [ '/wp-content/subsite/wp-admin/', '/wp-content/subsite/wp-content/' ];
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/wp-content/subsite/', excludedPaths ) );
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/wp-content/subsite/sample-page/', excludedPaths ) );
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-content/subsite/wp-admin/', excludedPaths ) );
+
+		// Without excluded paths, the legacy hardcoded paths are matched.
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-admin/', undefined ) );
+		assert.ok( wp.customize.utils.isExcludedPath( '/blog/wp-content/', undefined ), 'legacy fallback matches anywhere in the path' );
+		assert.ok( ! wp.customize.utils.isExcludedPath( '/sample-page/', undefined ) );
+		assert.ok( wp.customize.utils.isExcludedPath( '/wp-admin/', [] ), 'empty list falls back to legacy paths' );
+	} );
 });